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N-Acetyl-β-D-Glucosaminidase Assay Kit (Rate Method)
The N-Acetyl-β-D-Glucosaminidase Assay Kit is composed of Reagent R1, Reagent R2, and a calibrator. Reagent R1 contains a sodium phosphate buffer, while Reagent R2 includes a sodium phosphate buffer and 6-Methyl-2-Mercaptopyridine-N-Acetyl-β-D-Glucosaminide (MPT-NAG). The calibrator consists of N-Acetyl-β-D-Glucosaminidase (NAG) and sodium phosphate. This kit is designed for the quantitative analysis of NAG activity in human urine, primarily used to assess renal tubular damage.
Product English Name:
N-Acetyl-β-D-Glucosaminidase Assay Kit (Rate Method)
Alias:
NAG Assay Kit,β-NAG Test Kit
Intended Use:
This kit is used for the in vitro quantitative analysis of N-Acetyl-β-D-Glucosaminidase activity in human urine. Clinically, it is primarily employed to assist in the evaluation of renal tubular damage, providing critical insights into kidney health and function.
Product Introduction

The N-Acetyl-β-D-Glucosaminidase Assay Kit (Rate Method) is a specialized diagnostic tool designed for the precise measurement of NAG enzyme activity in human urine. This enzyme is a key biomarker for renal tubular damage, making the kit an essential component in clinical laboratories focused on kidney health. The kit includes all necessary reagents and a calibrator to ensure accurate and reliable results.

1. The kit features a highly sensitive and specific rate method for detecting NAG activity, ensuring precise measurements even at low concentrations.
2. It is composed of two main reagents: Reagent R1, which contains a sodium phosphate buffer, and Reagent R2, which includes a sodium phosphate buffer and MPT-NAG. The calibrator, containing NAG and sodium phosphate, ensures the accuracy of the assay.
3. The primary use of this kit is in clinical laboratories for the quantitative analysis of NAG activity in urine, aiding in the diagnosis and monitoring of renal tubular damage.

The N-Acetyl-β-D-Glucosaminidase Assay Kit, also known as the NAG Assay Kit, is a vital tool in clinical diagnostics. Its precise measurement capabilities and comprehensive composition make it indispensable for assessing renal health. Whether you're a seasoned clinician or a laboratory technician, this kit offers the reliability and accuracy needed for effective patient care.

Main Features

The N-Acetyl-β-D-Glucosaminidase Assay Kit offers a range of features designed to ensure accurate and reliable measurement of NAG activity in urine. Its primary functions include high sensitivity, specificity, and ease of use, making it a preferred choice in clinical settings.

1. High Sensitivity: The kit is capable of detecting low levels of NAG activity, ensuring accurate results even in early stages of renal damage.
2. Specificity: The rate method used in the kit is highly specific to NAG, minimizing the risk of cross-reactivity with other enzymes.
3. Ease of Use: The kit includes all necessary reagents and a detailed protocol, making it straightforward for laboratory technicians to perform the assay.
4. Calibration: The included calibrator ensures that each assay is accurate and reliable, providing consistent results across different batches.
5. Clinical Relevance: The kit is specifically designed for use in clinical laboratories, providing critical data for the assessment of renal tubular damage.

The N-Acetyl-β-D-Glucosaminidase Assay Kit stands out for its high sensitivity, specificity, and ease of use. These features make it an invaluable tool in clinical diagnostics, particularly for assessing renal health. Whether you're diagnosing early-stage kidney damage or monitoring ongoing conditions, this kit delivers the precision and reliability you need.

Clinical Applications

The N-Acetyl-β-D-Glucosaminidase Assay Kit is widely used in clinical settings for the quantitative analysis of NAG activity in urine. Its primary application is in the assessment of renal tubular damage, providing critical data for diagnosis and treatment planning.

1. Diagnosis of Renal Tubular Damage: The kit is used to detect elevated levels of NAG in urine, a key indicator of renal tubular damage.
2. Monitoring Kidney Health: Regular use of the kit allows clinicians to monitor the progression of kidney disease and the effectiveness of treatment.
3. Early Detection: The high sensitivity of the kit enables the early detection of renal damage, facilitating timely intervention.
4. Research Applications: Beyond clinical use, the kit is also employed in research studies focused on kidney health and disease.
5. Patient Management: The data provided by the kit aids in the management of patients with kidney disease, guiding treatment decisions and improving outcomes.

The N-Acetyl-β-D-Glucosaminidase Assay Kit is a cornerstone in the clinical assessment of renal health. Its applications range from early detection of kidney damage to ongoing patient management, making it an essential tool for clinicians and researchers alike. With its precise measurements and reliable results, this kit plays a crucial role in improving patient outcomes.

Purchasing Guide

When purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use. This guide provides essential information to help you make an informed decision.

1. Product Specifications: Ensure the kit meets your laboratory's requirements, including sensitivity, specificity, and ease of use.
2. Product Types: Consider the different types of kits available, such as those with varying reagent volumes or additional features.
3. Budget: Evaluate the cost of the kit in relation to your laboratory's budget, ensuring you get the best value for your investment.
4. Intended Use: Determine the specific clinical or research applications for which the kit will be used, ensuring it aligns with your needs.

Purchasing the N-Acetyl-β-D-Glucosaminidase Assay Kit requires careful consideration of various factors, from product specifications to budget. By taking the time to evaluate these aspects, you can ensure that you select the right kit for your laboratory's needs, ultimately enhancing the quality of your diagnostic and research efforts.

 Precautions

When using the N-Acetyl-β-D-Glucosaminidase Assay Kit, it is important to follow specific precautions to ensure accurate results and maintain safety. These include proper handling of reagents, calibration, and adherence to protocols.

1. Reagent Handling: Always handle reagents with care, ensuring they are stored at the recommended temperature and used within their expiration date.
2. Calibration: Regularly calibrate the assay using the provided calibrator to maintain accuracy and reliability.
3. Protocol Adherence: Follow the assay protocol precisely, including incubation times and reagent volumes, to ensure consistent results.
4. Safety Measures: Wear appropriate personal protective equipment, such as gloves and safety goggles, when handling reagents and performing the assay.
5. Waste Disposal: Dispose of used reagents and other materials according to laboratory safety guidelines to prevent contamination and ensure safety.

Using the N-Acetyl-β-D-Glucosaminidase Assay Kit requires attention to detail and adherence to safety protocols. By following these precautions, you can ensure the accuracy of your results and maintain a safe laboratory environment. Whether you're a seasoned technician or new to the assay, these guidelines will help you achieve reliable outcomes every time.

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