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N-Acetyl-β-D-Glucosaminidase Detection Kit (MPT Substrate Method)
The N-Acetyl-β-D-Glucosaminidase Detection Kit is composed of two main reagents. Reagent 1 contains a citric acid buffer at pH 7.5 and ProClin-300 at a concentration of 10 mmol/L and 0.3 ml/L, respectively. Reagent 2 also contains a citric acid buffer at pH 7.5, along with Methyl-2-Pyridyl 2-(Acetylamino)-2-Deoxy-1-Thio-BETA-D-Glucopyranoside (MPT-NAG), ProClin-300, and Tween-20 at concentrations of 10 mmol/L, 15 mmol/L, 0.3 ml/L, and 5 ml/L, respectively.
Product English Name:
N-Acetyl-β-D-Glucosaminidase Detection Kit (MPT Substrate Method)
Alias:
NAG Detection Kit,MPT-NAG Kit
Intended Use:
This product is designed for the quantitative in vitro detection of N-Acetyl-β-D-Glucosaminidase (NAG) levels in human urine. It is widely used in clinical laboratories to assess renal function and detect early signs of kidney damage.
Product Introduction

The N-Acetyl-β-D-Glucosaminidase Detection Kit (MPT Substrate Method) is a highly sensitive and specific diagnostic tool used to measure NAG enzyme activity in urine samples. This enzyme is a key biomarker for renal tubular damage, making the kit essential for early detection and monitoring of kidney-related diseases. The kit employs a colorimetric method, ensuring accurate and reliable results.

1. The kit is characterized by its high sensitivity and specificity, ensuring precise detection of NAG levels even at low concentrations. The use of MPT as a substrate enhances the accuracy of the assay.
2. The kit consists of two reagents: Reagent 1, which contains a citric acid buffer and ProClin-300, and Reagent 2, which includes MPT-NAG, ProClin-300, and Tween-20. These components work together to provide a stable and effective reaction environment.
3. The primary use of the kit is to detect NAG levels in human urine, which is crucial for assessing renal function. It is particularly useful in diagnosing early-stage kidney damage and monitoring the progression of kidney diseases.

The N-Acetyl-β-D-Glucosaminidase Detection Kit, also known as the NAG Detection Kit or MPT-NAG Kit, is a vital tool in clinical diagnostics. Its high sensitivity and specificity make it indispensable for detecting early signs of kidney damage. The kit's composition, including its two main reagents, ensures accurate and reliable results, making it a trusted choice for healthcare professionals.

Main Features

The N-Acetyl-β-D-Glucosaminidase Detection Kit offers high sensitivity, specificity, and reliability in detecting NAG levels in urine. It is designed for ease of use in clinical laboratories, providing accurate results for renal function assessment.

1. High Sensitivity: The kit can detect low concentrations of NAG, making it suitable for early detection of kidney damage.
2. Specificity: The use of MPT as a substrate ensures that the assay is specific to NAG, reducing the risk of false positives.
3. Stability: The reagents are formulated to maintain stability over a wide range of storage conditions, ensuring consistent performance.
4. Ease of Use: The kit is designed for straightforward application in clinical laboratories, with clear instructions and minimal preparation required.
5. Reliability: The colorimetric method used in the kit provides consistent and reproducible results, making it a reliable tool for renal function assessment.

The N-Acetyl-β-D-Glucosaminidase Detection Kit stands out for its high sensitivity, specificity, and reliability. Its ease of use and stability make it a preferred choice for clinical laboratories. The kit's ability to detect low NAG concentrations ensures early and accurate diagnosis of kidney-related conditions, making it an essential tool in renal health monitoring.

Clinical Applications

The N-Acetyl-β-D-Glucosaminidase Detection Kit is widely used in clinical settings to assess renal function and detect early signs of kidney damage. It is particularly valuable in diagnosing and monitoring kidney diseases.

1. Renal Function Assessment: The kit is used to measure NAG levels in urine, providing critical information about renal tubular health.
2. Early Detection of Kidney Damage: Elevated NAG levels are an early indicator of kidney damage, making the kit essential for early diagnosis.
3. Monitoring Disease Progression: The kit is used to monitor the progression of kidney diseases, helping healthcare providers adjust treatment plans accordingly.
4. Research Applications: The kit is also used in research studies to investigate the role of NAG in various kidney-related conditions.
5. Clinical Trials: The kit is employed in clinical trials to evaluate the efficacy of new treatments for kidney diseases.

The N-Acetyl-β-D-Glucosaminidase Detection Kit is a versatile tool in clinical practice, used for renal function assessment, early detection of kidney damage, and monitoring disease progression. Its applications extend to research and clinical trials, making it a valuable asset in the fight against kidney diseases.

Purchasing Guide

When purchasing the N-Acetyl-β-D-Glucosaminidase Detection Kit, consider factors such as product specifications, types, budget, and intended use to ensure the best fit for your clinical needs.

1. Product Specifications: Review the kit's sensitivity, specificity, and stability to ensure it meets your laboratory's requirements.
2. Product Types: Choose between different kit formats (e.g., single-use vs. bulk) based on your laboratory's volume and usage patterns.
3. Budget: Consider the cost per test and overall budget when selecting a kit, ensuring it provides good value for money.
4. Intended Use: Ensure the kit is suitable for your specific clinical applications, such as renal function assessment or research studies.

Purchasing the N-Acetyl-β-D-Glucosaminidase Detection Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can select a kit that best meets your clinical needs and ensures accurate and reliable results.

 Precautions

Proper handling and storage of the N-Acetyl-β-D-Glucosaminidase Detection Kit are essential to ensure accurate results and maintain reagent stability. Follow all safety guidelines and manufacturer instructions.

1. Storage Conditions: Store the kit at the recommended temperature to maintain reagent stability and performance.
2. Handling: Use proper laboratory techniques when handling reagents to avoid contamination and ensure accurate results.
3. Safety: Wear appropriate personal protective equipment (PPE) when working with the kit to protect against potential hazards.
4. Disposal: Dispose of used reagents and materials according to local regulations and laboratory safety protocols.
5. Calibration: Regularly calibrate equipment used with the kit to ensure consistent and accurate results.

The N-Acetyl-β-D-Glucosaminidase Detection Kit requires careful handling and storage to ensure accurate and reliable results. By following proper safety protocols, storage conditions, and disposal methods, you can maintain the integrity of the kit and protect laboratory personnel. Regular calibration of equipment is also essential to ensure consistent performance.

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