The 17α-Hydroxyprogesterone Assay Kit is a sophisticated diagnostic tool used in neonatal screening programs. It employs time-resolved fluorescence technology to accurately measure 17α-hydroxyprogesterone levels in dried blood spots. This kit is crucial for the early detection of congenital adrenal hyperplasia (CAH), a genetic disorder that affects adrenal gland function. The kit's components are meticulously designed to ensure high sensitivity and specificity, making it a reliable choice for clinical laboratories.

1. The kit features a highly sensitive time-resolved fluorescence method, which allows for the precise detection of 17α-hydroxyprogesterone levels in neonatal blood samples. This method is known for its low background noise and high signal-to-noise ratio, ensuring accurate results.
2. The kit's composition includes a range of essential components such as calibrators, controls, and specific reagents like the europium-labeled 17-OHP tracer and rabbit polyclonal antiserum. These components work together to provide a robust and reliable assay system.
3. The primary use of this kit is in neonatal screening for congenital adrenal hyperplasia (CAH). Early detection of CAH is critical for initiating timely treatment, which can prevent severe complications such as salt-wasting crises and ambiguous genitalia in affected infants.

The 17α-Hydroxyprogesterone Assay Kit, also known as the DELFIA Neonatal 17α-OH-progesterone Kit, is a vital tool in neonatal screening. Its advanced time-resolved fluorescence technology ensures accurate and reliable results, making it indispensable for the early detection of congenital adrenal hyperplasia. The kit's comprehensive components and precise methodology underscore its importance in clinical diagnostics.

The main features of the 17α-Hydroxyprogesterone Assay Kit include its high sensitivity, specificity, and reliability. It utilizes time-resolved fluorescence technology to accurately measure 17α-hydroxyprogesterone levels in neonatal blood samples, making it an essential tool for early detection of congenital adrenal hyperplasia.

1. High Sensitivity: The kit's time-resolved fluorescence method ensures low background noise and high signal-to-noise ratio, allowing for the detection of even low concentrations of 17α-hydroxyprogesterone.
2. Specificity: The use of europium-labeled 17-OHP tracer and rabbit polyclonal antiserum ensures that the assay is highly specific for 17α-hydroxyprogesterone, minimizing cross-reactivity with other compounds.
3. Reliability: The kit includes calibrators and controls that help maintain assay accuracy and precision over time, ensuring consistent and reliable results.
4. Ease of Use: The kit is designed for ease of use in clinical laboratories, with pre-prepared reagents and clear instructions that streamline the assay process.
5. Comprehensive Components: The kit contains all necessary components, including wash concentrate, assay buffer, and enhancement solution, ensuring a complete and efficient assay system.

The 17α-Hydroxyprogesterone Assay Kit stands out for its high sensitivity, specificity, and reliability. Its advanced time-resolved fluorescence technology, combined with a comprehensive set of components, makes it an indispensable tool for neonatal screening. The kit's ease of use and consistent performance further enhance its value in clinical diagnostics.

The 17α-Hydroxyprogesterone Assay Kit is primarily used in neonatal screening programs to detect congenital adrenal hyperplasia (CAH). Early detection of CAH is crucial for initiating timely treatment and preventing severe health complications.

1. Neonatal Screening: The kit is widely used in neonatal screening programs to detect elevated levels of 17α-hydroxyprogesterone, which is indicative of congenital adrenal hyperplasia (CAH).
2. Early Diagnosis: Early diagnosis of CAH allows for prompt treatment, which can prevent life-threatening salt-wasting crises and other severe complications.
3. Monitoring Treatment: The kit can also be used to monitor the effectiveness of treatment in infants diagnosed with CAH, ensuring that hormone levels are maintained within a normal range.
4. Research Applications: In addition to clinical use, the kit is also utilized in research studies to investigate the pathophysiology of CAH and other adrenal disorders.
5. Quality Control: The kit's inclusion of calibrators and controls makes it an excellent tool for quality control in clinical laboratories, ensuring the accuracy and reliability of assay results.

The 17α-Hydroxyprogesterone Assay Kit plays a critical role in neonatal screening for congenital adrenal hyperplasia. Its ability to provide accurate and reliable results ensures early diagnosis and effective treatment, ultimately improving outcomes for affected infants. The kit's versatility also extends to research and quality control applications, further highlighting its importance in the field of clinical diagnostics.

When purchasing the 17α-Hydroxyprogesterone Assay Kit, it is important to consider factors such as product specifications, types, budget, and intended use. This guide provides essential information to help you make an informed decision.

1. Product Specifications: Ensure that the kit meets your laboratory's specific requirements, including sensitivity, specificity, and compatibility with your existing equipment.
2. Product Types: The kit is available in different configurations, such as single-use or multi-use formats. Choose the type that best suits your laboratory's workflow and testing volume.
3. Budget: Consider the cost of the kit, including any additional reagents or consumables that may be required. Ensure that the kit fits within your laboratory's budget without compromising on quality.
4. Intended Use: Determine the primary use of the kit, whether for routine neonatal screening, research, or quality control. This will help you select the most appropriate kit for your needs.

Purchasing the 17α-Hydroxyprogesterone Assay Kit requires careful consideration of product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that you select the most suitable kit for your laboratory's needs, ultimately enhancing the accuracy and efficiency of your diagnostic processes.

When using the 17α-Hydroxyprogesterone Assay Kit, it is important to follow all safety and handling precautions to ensure accurate results and prevent contamination or injury.

1. Handling Reagents: Always handle reagents with care, wearing appropriate personal protective equipment (PPE) such as gloves and lab coats. Avoid contact with skin and eyes.
2. Storage Conditions: Store the kit components at the recommended temperatures to maintain their stability and effectiveness. Improper storage can lead to degraded performance.
3. Contamination Prevention: Use sterile techniques when handling samples and reagents to prevent contamination, which can affect assay results.
4. Equipment Calibration: Ensure that all equipment used in the assay, such as pipettes and microplate readers, are properly calibrated to ensure accurate measurements.
5. Waste Disposal: Dispose of all used reagents and samples according to local regulations and laboratory protocols to prevent environmental contamination and ensure safety.

Using the 17α-Hydroxyprogesterone Assay Kit requires strict adherence to safety and handling precautions. Proper handling of reagents, storage conditions, contamination prevention, equipment calibration, and waste disposal are all critical to ensuring accurate and reliable results. By following these precautions, you can maintain the integrity of your assay and protect the safety of laboratory personnel.