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EGFR/KRAS/ALK Gene Mutation Combined Detection Kit (Combined Probe Anchored Polymerization Sequencing Method)
The kit includes enzymes and buffers for DNA fragmentation and end repair, ligation, PCR amplification, hybridization, and purification. It also contains positive and negative controls, adapters, magnetic beads for purification, and wash and elution buffers. The components are designed to ensure accurate and efficient detection of gene mutations in non-small cell lung cancer (NSCLC) samples.
Product English Name:
EGFR/KRAS/ALK Gene Mutation Combined Detection Kit (Combined Probe Anchored Polymerization Sequencing Method)
Alias:
Multiplex Gene Mutation Detection Kit,Lung Cancer Mutation Detection Kit
Intended Use:
This kit is used for the qualitative detection of mutations in the EGFR, KRAS, and ALK genes in formalin-fixed paraffin-embedded (FFPE) tissue samples from clinically diagnosed NSCLC patients. It identifies specific mutations such as EGFR L858R, T790M, G719X, exon 19 deletions, KRAS G12D, and ALK fusions, which are critical for guiding targeted therapy decisions.
Product Introduction

The EGFR/KRAS/ALK Gene Mutation Combined Detection Kit is a cutting-edge diagnostic tool designed to identify key genetic mutations in non-small cell lung cancer (NSCLC) patients. Utilizing the Combined Probe Anchored Polymerization Sequencing Method, this kit offers high sensitivity and specificity in detecting mutations that influence treatment strategies, such as EGFR, KRAS, and ALK alterations.

1. The kit features advanced sequencing technology, ensuring precise detection of multiple gene mutations simultaneously. Its high-throughput design allows for efficient processing of FFPE samples, making it suitable for clinical laboratories.
2. The components include enzymes, buffers, and magnetic beads optimized for DNA fragmentation, ligation, PCR amplification, and hybridization. These elements work together to ensure reliable and reproducible results.
3. The kit is specifically designed for use in NSCLC patients, aiding in the selection of targeted therapies like gefitinib, icotinib, and crizotinib. It supports personalized medicine by identifying actionable mutations.

The EGFR/KRAS/ALK Gene Mutation Combined Detection Kit, also known as the Multiplex Gene Mutation Detection Kit, is a vital tool in modern oncology. It combines advanced sequencing technology with a comprehensive set of reagents to detect critical mutations in NSCLC patients, enabling tailored treatment plans and improving patient outcomes.

Main Features

This kit provides a comprehensive solution for detecting key gene mutations in NSCLC, offering high accuracy, efficiency, and ease of use in clinical settings.

1. High Sensitivity: The kit detects low-frequency mutations with high precision, ensuring reliable results even in samples with limited DNA.
2. Multiplex Detection: It simultaneously identifies mutations in EGFR, KRAS, and ALK genes, reducing the need for multiple tests.
3. Optimized Workflow: The included enzymes, buffers, and magnetic beads streamline the detection process, minimizing hands-on time.
4. Clinical Relevance: The detected mutations are directly linked to targeted therapies, aiding in treatment decision-making.
5. Quality Control: Positive and negative controls ensure the reliability and consistency of results across different batches.

The EGFR/KRAS/ALK Gene Mutation Combined Detection Kit stands out for its high sensitivity, multiplex capabilities, and clinical relevance. It simplifies the detection of critical mutations, empowering clinicians to make informed treatment decisions for NSCLC patients.

Clinical Applications

This kit is widely used in clinical settings to identify actionable mutations in NSCLC patients, guiding the selection of targeted therapies and improving treatment outcomes.

1. Diagnosis: The kit is used to confirm the presence of EGFR, KRAS, and ALK mutations in NSCLC patients, aiding in accurate diagnosis.
2. Treatment Selection: It helps clinicians choose appropriate targeted therapies, such as gefitinib for EGFR mutations or crizotinib for ALK fusions.
3. Prognosis: Identifying specific mutations can provide insights into disease progression and patient prognosis.
4. Clinical Trials: The kit is used to screen patients for eligibility in clinical trials testing new targeted therapies.
5. Research: It supports research into the molecular mechanisms of NSCLC and the development of new treatments.

The EGFR/KRAS/ALK Gene Mutation Combined Detection Kit plays a crucial role in clinical practice, from diagnosis to treatment selection and research. Its ability to identify key mutations ensures that NSCLC patients receive the most effective therapies available.

Purchasing Guide

This guide provides essential information for purchasing the EGFR/KRAS/ALK Gene Mutation Combined Detection Kit, including product specifications, types, budget considerations, and usage scenarios.

1. Product Parameters: The kit includes specific enzymes, buffers, and controls designed for high accuracy and efficiency in mutation detection.
2. Product Types: Available in different formats to suit various laboratory needs, including high-throughput and standard versions.
3. Budget Considerations: The kit offers excellent value for money, considering its comprehensive components and clinical utility.
4. Usage Scenarios: Ideal for clinical laboratories, research institutions, and hospitals focusing on NSCLC diagnosis and treatment.

When purchasing the EGFR/KRAS/ALK Gene Mutation Combined Detection Kit, consider its technical specifications, versatility, and cost-effectiveness. It is a valuable investment for any institution involved in NSCLC research or patient care.

 Precautions

Proper handling and usage of the EGFR/KRAS/ALK Gene Mutation Combined Detection Kit are essential to ensure accurate results and maintain product integrity.

1. Storage: Store the kit components at the recommended temperatures to preserve their functionality.
2. Handling: Use aseptic techniques to avoid contamination, which could compromise the results.
3. Sample Preparation: Ensure FFPE samples are properly prepared and meet the required quality standards.
4. Reagent Usage: Follow the instructions for reagent preparation and usage to achieve optimal performance.
5. Waste Disposal: Dispose of used reagents and samples according to local regulations to ensure safety and compliance.

To maximize the performance of the EGFR/KRAS/ALK Gene Mutation Combined Detection Kit, adhere to proper storage, handling, and usage guidelines. Attention to detail in sample preparation and reagent usage will ensure reliable and accurate results.

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