The D3-Hydroxybutyrate Detection Kit (Enzymatic Method) is a sophisticated diagnostic tool used to measure the concentration of D3-hydroxybutyrate, a key ketone body, in human serum or plasma. This kit employs an enzymatic method that ensures high specificity and sensitivity, making it an essential tool in clinical laboratories for diagnosing and monitoring metabolic conditions.

1. The kit features a highly specific enzymatic reaction that targets D3-hydroxybutyrate, ensuring accurate and reliable results. The use of D3-hydroxybutyrate dehydrogenase and diaphorase in Reagent 1 facilitates the conversion of D3-hydroxybutyrate to acetoacetate, which is then measured spectrophotometrically.
2. The composition of the kit includes two main reagents: Reagent 1 and Reagent 2. Reagent 1 contains Tris buffer, D3-hydroxybutyrate dehydrogenase, and diaphorase, while Reagent 2 includes Tris buffer, NAD+, INT, ammonium oxalate, and glutathione. These components work together to ensure the stability and accuracy of the assay.
3. The primary use of this kit is for the quantitative determination of D3-hydroxybutyrate levels in human serum or plasma. It is particularly useful in clinical settings for monitoring patients with diabetic ketoacidosis, where elevated levels of ketone bodies are a critical indicator of metabolic imbalance.

The D3-Hydroxybutyrate Detection Kit, also known as the D3-HB Kit, is a vital tool in clinical diagnostics. Its enzymatic method ensures precise measurement of ketone bodies, making it indispensable for managing metabolic disorders. The kit's robust composition and specific reagents guarantee reliable results, aiding healthcare professionals in making informed decisions.

The D3-Hydroxybutyrate Detection Kit offers high specificity and sensitivity in measuring D3-hydroxybutyrate levels. Its enzymatic method ensures accurate results, making it a reliable tool for clinical diagnostics.

1. The kit utilizes a highly specific enzymatic reaction to measure D3-hydroxybutyrate levels, ensuring accurate and reliable results.
2. The composition of the kit includes two main reagents, each containing specific enzymes and cofactors that facilitate the enzymatic reaction.
3. The kit is designed for use in clinical laboratories, providing a quick and efficient method for measuring ketone body levels in human serum or plasma.
4. The kit includes a calibrator and a control, which are essential for ensuring the accuracy and reliability of the assay.
5. The enzymatic method used in the kit is highly sensitive, allowing for the detection of low levels of D3-hydroxybutyrate, which is crucial for early diagnosis and monitoring of metabolic disorders.

The D3-Hydroxybutyrate Detection Kit stands out for its high specificity and sensitivity. Its enzymatic method ensures precise measurement of ketone bodies, making it an essential tool in clinical diagnostics. The kit's robust design and reliable performance make it a trusted choice for healthcare professionals.

The D3-Hydroxybutyrate Detection Kit is widely used in clinical settings for the diagnosis and monitoring of metabolic disorders, particularly diabetic ketoacidosis.

1. The kit is used in clinical laboratories to measure D3-hydroxybutyrate levels in patients with suspected diabetic ketoacidosis, providing critical information for diagnosis and treatment.
2. It is also used in the management of other metabolic disorders where ketone body levels are elevated, such as starvation ketosis and alcoholic ketoacidosis.
3. The kit is employed in emergency settings to quickly assess the severity of metabolic imbalances in critically ill patients.
4. It is used in research settings to study the role of ketone bodies in various metabolic processes and diseases.
5. The kit is also used in routine health check-ups to monitor ketone body levels in patients with diabetes, helping to prevent complications associated with elevated ketone levels.

The D3-Hydroxybutyrate Detection Kit is a versatile tool in clinical diagnostics, widely used for the diagnosis and monitoring of metabolic disorders. Its ability to provide quick and accurate results makes it invaluable in both emergency and routine clinical settings.

When purchasing the D3-Hydroxybutyrate Detection Kit, it is important to consider product specifications, types, budget, and intended use to ensure the best fit for your clinical needs.

1. Product specifications: Ensure the kit meets the required sensitivity and specificity for your clinical applications. Check the shelf life and storage conditions to ensure the kit remains effective over time.
2. Product types: There are different types of kits available, including those designed for high-throughput laboratories and those for smaller, more specialized labs. Choose the type that best fits your laboratory's workflow and volume.
3. Budget: Consider the cost of the kit, including any additional reagents or controls that may be required. Ensure that the kit fits within your laboratory's budget without compromising on quality.
4. Intended use: Determine the primary use of the kit in your laboratory, whether for routine testing, emergency diagnostics, or research purposes. This will help you select the most appropriate kit for your needs.

Purchasing the D3-Hydroxybutyrate Detection Kit requires careful consideration of product specifications, types, budget, and intended use. By taking these factors into account, you can ensure that the kit you choose will meet your clinical needs and provide reliable results.

Proper handling and storage of the D3-Hydroxybutyrate Detection Kit are essential to ensure accurate results and maintain the integrity of the reagents.

1. Storage: Store the kit at the recommended temperature to maintain the stability of the reagents. Avoid exposure to extreme temperatures or direct sunlight.
2. Handling: Use aseptic techniques when handling the reagents to prevent contamination. Ensure that all equipment used is clean and free from contaminants.
3. Calibration: Regularly calibrate the kit using the provided calibrator to ensure accurate and consistent results.
4. Disposal: Dispose of used reagents and materials according to local regulations and guidelines for biohazardous waste.
5. Training: Ensure that all personnel using the kit are properly trained in its use and understand the importance of following the protocol precisely.

The D3-Hydroxybutyrate Detection Kit requires careful handling and storage to ensure accurate results. Proper training, calibration, and disposal practices are essential to maintain the integrity of the kit and ensure reliable performance in clinical settings.