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1,5-Anhydro-D-Sorbitol Assay Kit (Pyranose Oxidase Method)
The 1,5-Anhydro-D-Sorbitol Assay Kit is composed of two main reagents: Reagent 1 (R1) and Reagent 2 (R2). R1 contains hexokinase, ATP, magnesium chloride, N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline sodium salt, phosphoenolpyruvate, and pyruvate kinase. R2 includes pyranose oxidase, 4-aminoantipyrine, and horseradish peroxidase. The kit also includes a calibrator and control samples with specified concentration ranges.
Product English Name:
1,5-Anhydro-D-Sorbitol Assay Kit (Pyranose Oxidase Method)
Alias:
1,5-AG Assay Kit,Sorbitol Test Kit
Intended Use:
This kit is designed for the quantitative determination of 1,5-anhydro-D-sorbitol (1,5-AG) in human serum or plasma. It is primarily used in clinical settings to monitor blood glucose levels, providing valuable insights for diabetes management and related conditions.
Product Introduction

The 1,5-Anhydro-D-Sorbitol Assay Kit is a specialized diagnostic tool used to measure the concentration of 1,5-AG in human serum or plasma. This kit employs the pyranose oxidase method, ensuring high accuracy and reliability. It is widely used in clinical laboratories to assist in the monitoring of blood glucose levels, particularly in diabetic patients.

1. The kit features a highly sensitive and specific enzymatic reaction, ensuring precise measurement of 1,5-AG levels. The inclusion of multiple enzymes and co-factors in the reagents enhances the reaction efficiency and accuracy.
2. The composition of the kit includes two main reagents, a calibrator, and control samples. Reagent 1 contains hexokinase and ATP, while Reagent 2 includes pyranose oxidase and horseradish peroxidase. The calibrator and control samples are designed to ensure the reliability of the test results.
3. The primary use of this kit is to monitor blood glucose levels in diabetic patients. By measuring 1,5-AG levels, healthcare providers can gain insights into short-term glycemic control, complementing traditional glucose monitoring methods.

The 1,5-Anhydro-D-Sorbitol Assay Kit, also known as the 1,5-AG Assay Kit, is a vital tool in clinical diagnostics. Its unique composition and precise enzymatic reactions make it an essential resource for monitoring blood glucose levels, particularly in diabetic patients. This kit not only provides accurate results but also enhances the overall understanding of glycemic control.

Main Features

The 1,5-Anhydro-D-Sorbitol Assay Kit offers high sensitivity and specificity in measuring 1,5-AG levels. Its enzymatic method ensures reliable and accurate results, making it a valuable tool in clinical diagnostics.

1. The kit utilizes a two-reagent system, with each reagent containing specific enzymes and co-factors that enhance the accuracy of the 1,5-AG measurement.
2. The inclusion of a calibrator and control samples ensures that the test results are reliable and consistent, providing confidence in the accuracy of the measurements.
3. The enzymatic reaction employed by the kit is highly specific to 1,5-AG, reducing the likelihood of interference from other substances in the serum or plasma.
4. The kit is designed for ease of use, with clear instructions and a straightforward protocol that can be easily followed by laboratory technicians.
5. The results obtained from this kit provide valuable insights into short-term glycemic control, complementing traditional glucose monitoring methods and offering a more comprehensive view of a patient's glucose management.

The 1,5-Anhydro-D-Sorbitol Assay Kit stands out for its precision and reliability. Its enzymatic method, combined with a well-designed reagent system, ensures accurate measurement of 1,5-AG levels. This kit is not only easy to use but also provides critical data for managing blood glucose levels, making it an indispensable tool in clinical settings.

Clinical Applications

The 1,5-Anhydro-D-Sorbitol Assay Kit is primarily used in clinical laboratories to monitor blood glucose levels in diabetic patients. It provides a reliable method for assessing short-term glycemic control.

1. The kit is used in the diagnosis and management of diabetes, providing a more comprehensive view of a patient's glucose control over a short period.
2. It is particularly useful in monitoring the effectiveness of diabetes treatment plans, allowing healthcare providers to make informed adjustments to therapy.
3. The kit can be used in research settings to study the relationship between 1,5-AG levels and various metabolic conditions, offering insights into the pathophysiology of diabetes.
4. In clinical trials, the kit is employed to evaluate the efficacy of new diabetes medications, providing a reliable measure of short-term glycemic control.
5. The kit is also used in routine health check-ups to assess the risk of developing diabetes, offering an early warning system for individuals with elevated 1,5-AG levels.

The 1,5-Anhydro-D-Sorbitol Assay Kit is a versatile tool in clinical practice, offering valuable insights into blood glucose management. Its applications range from routine diabetes monitoring to advanced research, making it an essential resource for healthcare providers and researchers alike.

Purchasing Guide

When purchasing the 1,5-Anhydro-D-Sorbitol Assay Kit, it is important to consider the product specifications, intended use, and budget. This guide provides essential information to help you make an informed decision.

1. Product Parameters: Ensure that the kit meets the required sensitivity and specificity for your intended use. Check the concentration ranges of the calibrator and control samples.
2. Product Types: The kit is available in different formats, including single-use and multi-use options. Choose the format that best suits your laboratory's needs.
3. Budget Considerations: Evaluate the cost-effectiveness of the kit, considering the number of tests it can perform and the overall value it provides.
4. Usage Scenarios: Consider the specific clinical or research applications for which the kit will be used. Ensure that the kit is compatible with your laboratory's equipment and protocols.

Purchasing the 1,5-Anhydro-D-Sorbitol Assay Kit requires careful consideration of various factors, including product specifications, budget, and intended use. By following this guide, you can ensure that you select the right kit for your needs, providing reliable and accurate results for your clinical or research applications.

 Precautions

When using the 1,5-Anhydro-D-Sorbitol Assay Kit, it is important to follow the instructions carefully to ensure accurate results. This section outlines key precautions to consider during the testing process.

1. Always use the kit within its expiration date to ensure the reliability of the results. Expired reagents may lead to inaccurate measurements.
2. Store the kit according to the manufacturer's instructions, typically at 2-8°C. Improper storage can affect the performance of the reagents.
3. Ensure that all equipment used in the testing process is clean and calibrated. Contaminated or improperly calibrated equipment can lead to erroneous results.
4. Follow the recommended sample preparation procedures carefully. Improper handling of serum or plasma samples can affect the accuracy of the test.
5. Always run control samples alongside patient samples to verify the accuracy of the test results. This step is crucial for ensuring the reliability of the measurements.

Using the 1,5-Anhydro-D-Sorbitol Assay Kit requires attention to detail and adherence to the recommended procedures. By following these precautions, you can ensure that the test results are accurate and reliable, providing valuable insights into blood glucose management.

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