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CD8+19 FITC/CD3+56 PE/CD45 PC5.5/CD4 PC7/MitoDye Detection Kit (Flow Cytometry)
This kit is composed of multicolor antibodies, probes, and lysing agents. The multicolor antibodies include CD8 FITC, CD19 FITC, CD3 PE, CD56 PE, CD45 PerCP-Cy5.5, and CD4 PE-Cyanine7. The phosphate buffer has a pH of 7.4 and contains 0.12% NaH2PO4, 0.88% NaCl, 0.2% gelatin, and 0.09% sodium azide. The fluorescent probe is MitoDye, and the lysing agent is a less than 10% ammonium chloride solution.
Product English Name:
CD8+19 FITC/CD3+56 PE/CD45 PC5.5/CD4 PC7/MitoDye Detection Kit (Flow Cytometry)
Alias:
Multicolor Flow Cytometry Kit,Lymphocyte Subset Detection Kit
Intended Use:
This product is used for the in vitro quantitative detection of the percentage of T lymphocytes and their subsets, B lymphocytes, and natural killer cells in human whole blood, as well as their metabolic state indicators.
Product Introduction

The CD8+19 FITC/CD3+56 PE/CD45 PC5.5/CD4 PC7/MitoDye Detection Kit is a state-of-the-art tool designed for flow cytometry analysis. It enables precise detection and quantification of various lymphocyte subsets and their metabolic states in human blood samples. This kit is essential for immunology research and clinical diagnostics, providing reliable and accurate results.

1. The kit features a combination of multicolor antibodies and a fluorescent probe, allowing for simultaneous detection of multiple cell markers. This multiplexing capability enhances the efficiency and accuracy of flow cytometry analysis.
2. The composition includes a carefully balanced phosphate buffer that ensures optimal pH and stability for the antibodies and probes. The lysing agent effectively removes red blood cells, leaving a pure sample for analysis.
3. This kit is specifically designed for the quantitative assessment of T lymphocytes, B lymphocytes, and natural killer cells, providing critical insights into immune system function and metabolic activity.

The CD8+19 FITC/CD3+56 PE/CD45 PC5.5/CD4 PC7/MitoDye Detection Kit is a versatile and reliable tool for flow cytometry. Its multicolor antibody panel and robust buffer system make it indispensable for immunology research and clinical diagnostics. Whether you're studying lymphocyte subsets or assessing metabolic states, this kit delivers precise and consistent results.

Main Features

This detection kit offers high sensitivity and specificity for identifying and quantifying lymphocyte subsets and their metabolic states. Its multicolor antibody panel and optimized buffer system ensure accurate and reproducible results.

1. The kit includes a comprehensive panel of multicolor antibodies, enabling simultaneous detection of multiple cell markers. This feature significantly reduces the time and effort required for complex immunophenotyping studies.
2. The phosphate buffer is specifically formulated to maintain antibody stability and ensure consistent performance across different experiments. Its balanced pH and composition prevent degradation and enhance signal clarity.
3. The fluorescent probe, MitoDye, is designed to assess cellular metabolic activity, providing valuable insights into the functional state of immune cells. This feature is particularly useful for studying immune responses and metabolic disorders.
4. The lysing agent effectively removes red blood cells without damaging the target lymphocytes, ensuring a clean and accurate sample for analysis. This step is crucial for obtaining reliable flow cytometry data.
5. The kit is compatible with a wide range of flow cytometry instruments, making it a versatile choice for both research and clinical laboratories. Its ease of use and consistent performance make it a preferred option for immunology studies.

The CD8+19 FITC/CD3+56 PE/CD45 PC5.5/CD4 PC7/MitoDye Detection Kit stands out for its comprehensive antibody panel, optimized buffer system, and reliable lysing agent. Whether you're conducting research or performing clinical diagnostics, this kit provides the tools you need for accurate and efficient flow cytometry analysis.

Clinical Applications

This kit is widely used in clinical and research settings for the analysis of lymphocyte subsets and their metabolic states. It is particularly valuable in immunology, oncology, and infectious disease studies.

1. In immunology research, the kit is used to study the distribution and function of T lymphocytes, B lymphocytes, and natural killer cells. This information is crucial for understanding immune responses and developing new therapies.
2. In oncology, the kit helps identify and quantify tumor-infiltrating lymphocytes, providing insights into the immune microenvironment of tumors. This data is essential for cancer immunotherapy research.
3. In infectious disease studies, the kit is used to monitor changes in lymphocyte subsets during infection and treatment. This information helps assess immune system recovery and treatment efficacy.
4. The kit is also used in clinical diagnostics to evaluate immune system function in patients with autoimmune diseases, immunodeficiencies, and other conditions. It provides critical data for patient management and treatment planning.
5. In metabolic research, the kit's MitoDye probe is used to assess cellular metabolic activity, offering insights into the metabolic state of immune cells. This feature is particularly useful for studying metabolic disorders and their impact on immune function.

The CD8+19 FITC/CD3+56 PE/CD45 PC5.5/CD4 PC7/MitoDye Detection Kit is a versatile tool with broad applications in immunology, oncology, infectious disease research, and clinical diagnostics. Its ability to provide detailed insights into lymphocyte subsets and metabolic states makes it an invaluable resource for researchers and clinicians alike.

Purchasing Guide

When purchasing this detection kit, consider factors such as product specifications, types, budget, and intended use. This guide provides essential information to help you make an informed decision.

1. Product specifications: Ensure the kit includes all necessary components, such as multicolor antibodies, a phosphate buffer, a fluorescent probe, and a lysing agent. Verify the compatibility of the kit with your flow cytometry instrument.
2. Product types: This kit is available in different configurations, such as single-use and bulk packages. Choose the type that best suits your laboratory's needs and workflow.
3. Budget: Consider the cost of the kit in relation to your research or clinical budget. While higher-priced kits may offer additional features, ensure they align with your specific requirements.
4. Intended use: Determine whether the kit will be used for research, clinical diagnostics, or both. Ensure the kit's features and performance meet the demands of your intended applications.

Purchasing the CD8+19 FITC/CD3+56 PE/CD45 PC5.5/CD4 PC7/MitoDye Detection Kit requires careful consideration of product specifications, types, budget, and intended use. By evaluating these factors, you can select the kit that best meets your laboratory's needs and ensures successful flow cytometry analysis.

 Precautions

Proper handling and storage of the detection kit are essential to ensure accurate and reliable results. Follow these precautions to maintain the integrity of the components.

1. Store the kit at the recommended temperature to prevent degradation of the antibodies and probes. Avoid exposure to light and moisture, which can affect performance.
2. Use aseptic techniques when handling the kit components to prevent contamination. Contaminated samples can lead to inaccurate results and compromise the integrity of your data.
3. Follow the manufacturer's instructions for preparing and using the kit. Deviating from the recommended protocol can affect the performance and reliability of the kit.
4. Dispose of used components according to local regulations and laboratory safety guidelines. Proper disposal ensures the safety of laboratory personnel and the environment.
5. Regularly calibrate and maintain your flow cytometry instrument to ensure accurate and consistent results. Proper instrument maintenance is crucial for the reliable performance of the detection kit.

The CD8+19 FITC/CD3+56 PE/CD45 PC5.5/CD4 PC7/MitoDye Detection Kit requires careful handling and storage to ensure optimal performance. By following the recommended precautions, you can maintain the integrity of the components and achieve accurate and reliable flow cytometry results.

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