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CD45/CD4/CD3 Detection Kit (Flow Cytometry)
The CD45/CD4/CD3 Detection Kit is composed of three main components: CD45-FITC, CD4-RD1, and CD3-PC5. These fluorescently labeled antibodies are designed to specifically bind to their respective antigens on human peripheral blood leukocytes, enabling precise identification and quantification of T-cell subsets using flow cytometry.
Product English Name:
CD45/CD4/CD3 Detection Kit (Flow Cytometry)
Alias:
T-Cell Detection Kit,Lymphocyte Subset Kit
Intended Use:
This kit is used to identify and count CD3+, CD4+, and CD3+/CD4+ double-positive lymphocytes in human peripheral blood samples. It is essential for immunophenotyping, monitoring immune responses, and diagnosing immune-related disorders.
Product Introduction

The CD45/CD4/CD3 Detection Kit is a specialized tool for flow cytometry, designed to analyze T-cell subsets in human peripheral blood. It utilizes fluorescently labeled antibodies to target specific cell surface markers, providing accurate and reliable data for immunology research and clinical diagnostics.

1. The kit features high specificity and sensitivity, ensuring precise detection of CD3, CD4, and CD45 markers. Its optimized formulation minimizes background noise and enhances signal clarity.
2. The kit consists of three key components: CD45-FITC, CD4-RD1, and CD3-PC5. These antibodies are conjugated to distinct fluorochromes, allowing simultaneous detection of multiple markers in a single sample.
3. It is primarily used for immunophenotyping, enabling researchers and clinicians to study T-cell populations, monitor immune responses, and diagnose conditions such as HIV/AIDS and autoimmune diseases.

The CD45/CD4/CD3 Detection Kit, also known as the T-Cell Detection Kit, is a powerful tool for analyzing lymphocyte subsets. Its precise components and robust performance make it indispensable for both research and clinical applications.

Main Features

This kit offers high-precision detection of T-cell subsets, enabling detailed analysis of immune cell populations. Its multi-color fluorescence design allows simultaneous detection of CD3, CD4, and CD45 markers.

1. High specificity: The antibodies are rigorously validated to ensure minimal cross-reactivity and accurate targeting of CD3, CD4, and CD45 markers.
2. Multi-color detection: The use of distinct fluorochromes (FITC, RD1, PC5) enables simultaneous analysis of multiple markers in a single sample.
3. Optimized for flow cytometry: The kit is designed to work seamlessly with flow cytometers, providing clear and reproducible results.
4. Reliable performance: The kit delivers consistent results across different sample types and experimental conditions.
5. Easy to use: The ready-to-use format simplifies the staining process, reducing preparation time and potential errors.

The CD45/CD4/CD3 Detection Kit stands out for its precision, versatility, and ease of use. Whether you're studying immune responses or diagnosing diseases, this kit delivers reliable and actionable insights.

Clinical Applications

This kit is widely used in clinical settings for immunophenotyping, diagnosing immune disorders, and monitoring treatment responses in conditions like HIV/AIDS and autoimmune diseases.

1. Immunophenotyping: The kit is essential for identifying and quantifying T-cell subsets, providing critical data for understanding immune system function.
2. HIV/AIDS monitoring: It is used to track CD4+ T-cell counts, a key indicator of disease progression and treatment efficacy.
3. Autoimmune disease diagnosis: The kit helps identify abnormal T-cell populations associated with autoimmune disorders.
4. Transplant monitoring: It is used to assess immune cell recovery and rejection risks in transplant patients.
5. Research applications: The kit supports studies on immune responses, vaccine development, and immunotherapy.

From diagnosing diseases to advancing research, the CD45/CD4/CD3 Detection Kit plays a vital role in clinical and scientific applications. Its versatility and reliability make it a trusted tool in immunology.

Purchasing Guide

When purchasing the CD45/CD4/CD3 Detection Kit, consider factors such as product specifications, intended use, budget, and compatibility with your flow cytometry system.

1. Product parameters: Ensure the kit includes the required antibodies (CD45-FITC, CD4-RD1, CD3-PC5) and verify their concentrations and fluorochrome compatibility.
2. Product types: Choose between research-grade and clinical-grade kits based on your application and regulatory requirements.
3. Budget considerations: Evaluate the cost per test and compare prices across suppliers to find the best value.
4. Usage scenarios: Consider the sample types (e.g., whole blood, PBMCs) and the specific markers you need to detect.

Purchasing the right CD45/CD4/CD3 Detection Kit requires careful consideration of your needs and budget. By focusing on key parameters and intended use, you can ensure optimal performance and value.

 Precautions

Proper handling and storage of the CD45/CD4/CD3 Detection Kit are essential to maintain its performance and ensure accurate results.

1. Storage conditions: Store the kit at 2-8°C and protect it from light to preserve antibody stability.
2. Sample preparation: Use fresh or properly preserved samples to avoid degradation of target markers.
3. Staining protocol: Follow the recommended staining procedure to ensure optimal antibody binding and minimize non-specific staining.
4. Instrument calibration: Regularly calibrate your flow cytometer to ensure accurate detection of fluorescence signals.
5. Waste disposal: Dispose of reagents and samples in accordance with local regulations for biohazardous materials.

Handling the CD45/CD4/CD3 Detection Kit with care is crucial for achieving reliable results. By adhering to storage, preparation, and disposal guidelines, you can maximize its performance and longevity.

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