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CD4-FITC/CD8-PE/CD3-PerCP Fluorescent Monoclonal Antibody Kit (Flow Cytometry Method)
The kit consists of CD4-FITC, CD8-PE, and CD3-PerCP fluorescent monoclonal antibodies dissolved in 1 mL of phosphate-buffered saline (pH 7.2) containing 0.2% BSA and 1‰ Proclin 300. It is designed for use with flow cytometry to analyze human T lymphocyte subsets in whole blood samples.
Product English Name:
CD4-FITC/CD8-PE/CD3-PerCP Fluorescent Monoclonal Antibody Kit (Flow Cytometry Method)
Alias:
T Cell Subset Antibody Kit,Flow Cytometry Antibody Kit
Intended Use:
This product is used in conjunction with flow cytometry to determine the percentage of mature T lymphocytes and their subsets in lysed whole blood samples. It specifically identifies T lymphocytes (CD3+), helper/inducer T lymphocytes (CD3+CD4+), and suppressor/cytotoxic T lymphocytes (CD3+CD8+).
Product Introduction

The CD4-FITC/CD8-PE/CD3-PerCP Fluorescent Monoclonal Antibody Kit is a specialized tool for flow cytometry, enabling precise identification and quantification of T lymphocyte subsets in human blood. It is widely used in immunology research and clinical diagnostics to assess immune system status and monitor diseases such as HIV/AIDS.

1. The kit features high specificity and sensitivity, ensuring accurate detection of T lymphocyte subsets. Its fluorescent labels (FITC, PE, and PerCP) allow for simultaneous multi-parameter analysis, making it a versatile tool for immunophenotyping.
2. The composition includes CD4-FITC, CD8-PE, and CD3-PerCP monoclonal antibodies suspended in a phosphate-buffered solution with stabilizers like BSA and Proclin 300. This formulation ensures stability and optimal performance during flow cytometry analysis.
3. Its primary use is to determine the percentage of T lymphocyte subsets in whole blood samples, aiding in the diagnosis and monitoring of immune-related conditions. It is particularly valuable in HIV/AIDS research, where CD4+ T cell counts are critical.

The CD4-FITC/CD8-PE/CD3-PerCP Fluorescent Monoclonal Antibody Kit, also known as the T Cell Subset Antibody Kit, is a powerful tool for flow cytometry. Its ability to accurately identify and quantify T lymphocyte subsets makes it indispensable in both research and clinical settings. Whether you're studying immune responses or monitoring disease progression, this kit delivers reliable and precise results.

Main Features

This kit is designed for high-precision analysis of T lymphocyte subsets using flow cytometry. Its key features include multi-parameter detection, high specificity, and ease of use.

1. Multi-parameter detection: The kit allows simultaneous analysis of CD3, CD4, and CD8 markers using distinct fluorescent labels (FITC, PE, and PerCP), enabling comprehensive immunophenotyping.
2. High specificity: The monoclonal antibodies are highly specific to their respective antigens, ensuring accurate identification of T lymphocyte subsets.
3. Stability: The antibodies are suspended in a phosphate-buffered solution with stabilizers, ensuring long-term stability and consistent performance.
4. User-friendly: The kit is designed for ease of use, with clear instructions and minimal preparation required, making it suitable for both novice and experienced users.
5. Versatility: It is compatible with a wide range of flow cytometry instruments, making it a flexible choice for various laboratory settings.

The CD4-FITC/CD8-PE/CD3-PerCP Fluorescent Monoclonal Antibody Kit stands out for its precision, versatility, and ease of use. Whether you're analyzing immune responses or diagnosing diseases, this kit provides reliable and accurate results, making it a valuable addition to any laboratory.

Clinical Applications

This kit is widely used in clinical and research settings to analyze T lymphocyte subsets, particularly in the diagnosis and monitoring of immune-related diseases such as HIV/AIDS.

1. HIV/AIDS monitoring: The kit is essential for measuring CD4+ T cell counts, a critical parameter in managing HIV/AIDS patients.
2. Immunodeficiency disorders: It helps diagnose and monitor conditions characterized by abnormal T lymphocyte levels, such as primary immunodeficiency disorders.
3. Autoimmune diseases: The kit aids in studying T lymphocyte subsets in autoimmune diseases like lupus and rheumatoid arthritis.
4. Cancer research: It is used to analyze T cell populations in cancer patients, particularly in immunotherapy studies.
5. Transplant monitoring: The kit helps assess immune reconstitution in patients undergoing hematopoietic stem cell transplantation.

The CD4-FITC/CD8-PE/CD3-PerCP Fluorescent Monoclonal Antibody Kit is a cornerstone in clinical diagnostics and research. Its ability to provide detailed insights into T lymphocyte subsets makes it invaluable for managing diseases like HIV/AIDS, studying autoimmune disorders, and advancing cancer immunotherapy.

Purchasing Guide

This guide provides essential information for purchasing the CD4-FITC/CD8-PE/CD3-PerCP Fluorescent Monoclonal Antibody Kit, including product specifications, types, budget considerations, and usage scenarios.

1. Product specifications: Ensure the kit includes CD4-FITC, CD8-PE, and CD3-PerCP antibodies in a phosphate-buffered solution with stabilizers. Verify the volume and concentration to meet your experimental needs.
2. Product types: Choose between standard and bulk kits based on your laboratory's requirements. Bulk kits are ideal for high-throughput studies.
3. Budget considerations: Compare prices from different suppliers, factoring in shipping costs and potential discounts for bulk purchases.
4. Usage scenarios: Consider the kit's compatibility with your flow cytometry instruments and its suitability for your specific research or clinical applications.

Purchasing the CD4-FITC/CD8-PE/CD3-PerCP Fluorescent Monoclonal Antibody Kit requires careful consideration of product specifications, types, and budget. By aligning your purchase with your laboratory's needs, you can ensure optimal performance and cost-effectiveness.

 Precautions

Proper handling and storage of the CD4-FITC/CD8-PE/CD3-PerCP Fluorescent Monoclonal Antibody Kit are essential to ensure accurate results and prolong its shelf life.

1. Storage: Store the kit at 2-8°C and protect it from light. Avoid freezing to prevent antibody degradation.
2. Handling: Use sterile techniques to avoid contamination. Allow the kit to reach room temperature before use.
3. Preparation: Follow the manufacturer's instructions for preparing and diluting the antibodies. Incorrect preparation can lead to inaccurate results.
4. Instrument calibration: Regularly calibrate your flow cytometer to ensure accurate detection of fluorescent signals.
5. Waste disposal: Dispose of used reagents and samples according to local biohazard waste regulations.

The CD4-FITC/CD8-PE/CD3-PerCP Fluorescent Monoclonal Antibody Kit requires careful handling and storage to maintain its performance. By following proper protocols, you can ensure reliable results and extend the kit's usability, making it a dependable tool for your laboratory.

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