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CD3/CD16+CD56/CD45/CD4/CD19/CD8 Detection Kit (Flow Cytometry Method)
This kit primarily includes mouse anti-human CD3, CD16+CD56, CD45, CD4, CD19, and CD8 six-color antibody reagents, along with a lysing solution. These components are designed for precise detection and analysis of lymphocyte subsets in human peripheral blood samples using flow cytometry.
Product English Name:
CD3/CD16+CD56/CD45/CD4/CD19/CD8 Detection Kit (Flow Cytometry Method)
Alias:
Lymphocyte Subset Detection Kit,Flow Cytometry Antibody Kit
Intended Use:
The kit is used for the quantitative in vitro detection of lymphocyte subsets in anticoagulated human peripheral blood samples. It identifies mature T lymphocytes (CD3+), helper/inducer T lymphocytes (CD3+CD4+), suppressor/cytotoxic T lymphocytes (CD3+CD8+), B lymphocytes (CD3-CD19+), and natural killer cells (CD3-CD16+CD56+).
Product Introduction

The CD3/CD16+CD56/CD45/CD4/CD19/CD8 Detection Kit is a state-of-the-art tool designed for flow cytometry analysis. It enables researchers and clinicians to accurately quantify and analyze various lymphocyte subsets in human peripheral blood. This kit is essential for immunophenotyping, immune monitoring, and research in immunology and hematology.

1. The kit features six-color antibody reagents, allowing simultaneous detection of multiple lymphocyte markers. This multiplexing capability enhances efficiency and reduces sample volume requirements.
2. The composition includes highly specific mouse anti-human antibodies targeting CD3, CD16+CD56, CD45, CD4, CD19, and CD8. These antibodies are optimized for flow cytometry, ensuring high sensitivity and specificity.
3. The primary use of this kit is in clinical diagnostics and research, particularly for immune system monitoring, disease diagnosis, and therapeutic evaluation. It provides detailed insights into lymphocyte populations, aiding in the understanding of immune responses.

The CD3/CD16+CD56/CD45/CD4/CD19/CD8 Detection Kit is a versatile and reliable tool for flow cytometry. Its six-color antibody reagents and lysing solution make it indispensable for immunophenotyping and immune monitoring. Whether in research or clinical settings, this kit offers precise and comprehensive analysis of lymphocyte subsets.

Main Features

This detection kit offers high sensitivity and specificity for identifying lymphocyte subsets. Its six-color antibody reagents enable simultaneous detection of multiple markers, streamlining the analysis process.

1. The kit includes antibodies for CD3, CD16+CD56, CD45, CD4, CD19, and CD8, providing comprehensive coverage of key lymphocyte subsets.
2. The six-color design allows for multiplexed detection, reducing the need for multiple assays and conserving sample volume.
3. The antibodies are highly specific, ensuring accurate identification and quantification of target cells.
4. The included lysing solution facilitates efficient red blood cell removal, enhancing the clarity of flow cytometry results.
5. The kit is optimized for use with flow cytometry instruments, ensuring compatibility and ease of use.

The CD3/CD16+CD56/CD45/CD4/CD19/CD8 Detection Kit stands out for its multiplexing capability, specificity, and ease of use. It simplifies the process of lymphocyte subset analysis, making it a valuable tool for both research and clinical applications.

Clinical Applications

This kit is widely used in clinical diagnostics and research for immune system monitoring, disease diagnosis, and therapeutic evaluation.

1. In clinical diagnostics, the kit is used to assess immune status and diagnose immune-related disorders such as HIV, autoimmune diseases, and lymphomas.
2. Researchers utilize the kit to study immune responses in various conditions, including infections, cancers, and post-transplant monitoring.
3. The kit aids in therapeutic evaluation by monitoring changes in lymphocyte subsets during treatment, helping to assess treatment efficacy.
4. It is also used in hematology to differentiate and quantify lymphocyte populations in blood samples.
5. The kit supports immunophenotyping studies, providing detailed insights into immune cell populations and their roles in health and disease.

The CD3/CD16+CD56/CD45/CD4/CD19/CD8 Detection Kit is a cornerstone in clinical and research settings. Its ability to provide detailed lymphocyte subset analysis makes it invaluable for diagnosing diseases, monitoring immune responses, and evaluating treatments.

Purchasing Guide

When purchasing this kit, consider product specifications, types, budget, and intended use to ensure it meets your needs.

1. Evaluate the product parameters, such as antibody specificity, fluorochrome compatibility, and lysing solution efficiency, to ensure they align with your experimental requirements.
2. Choose the appropriate kit type based on your flow cytometry instrument and the number of markers you need to detect simultaneously.
3. Set a budget that accounts for the kit's cost, as well as any additional reagents or equipment needed for your experiments.
4. Consider the intended use, whether for clinical diagnostics, research, or therapeutic monitoring, to select the most suitable kit version.

Purchasing the CD3/CD16+CD56/CD45/CD4/CD19/CD8 Detection Kit requires careful consideration of product specifications, types, and intended use. By aligning these factors with your needs, you can ensure optimal performance and results.

 Precautions

Proper handling and storage of the kit components are crucial to ensure accurate and reliable results.

1. Store the antibodies and lysing solution at the recommended temperature to maintain their stability and effectiveness.
2. Avoid repeated freeze-thaw cycles, as this can degrade the antibodies and affect performance.
3. Follow the manufacturer's instructions for sample preparation and staining to ensure consistent and accurate results.
4. Use appropriate controls and calibration beads to validate your flow cytometry results and ensure instrument performance.
5. Dispose of reagents and samples according to local regulations and safety guidelines to prevent contamination and ensure safety.

Handling the CD3/CD16+CD56/CD45/CD4/CD19/CD8 Detection Kit with care is essential for obtaining reliable results. Proper storage, handling, and disposal practices will help maintain the kit's performance and ensure accurate analysis of lymphocyte subsets.

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