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CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Fluorescent Monoclonal Antibody Kit (Flow Cytometry)
The CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Fluorescent Monoclonal Antibody Kit is designed for the precise identification and quantification of human mature lymphocyte subsets in whole blood samples. It includes a combination of monoclonal antibodies conjugated with different fluorescent dyes, enabling simultaneous detection of T lymphocytes (CD3+), helper/inducer T lymphocytes (CD3+CD4+), and suppressor/cytotoxic T lymphocytes (CD3+CD8+). This kit is widely used in immunology research and clinical diagnostics.
Product English Name:
CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Fluorescent Monoclonal Antibody Kit (Flow Cytometry)
Alias:
Flow Cytometry Antibody Kit,Lymphocyte Subset Detection Kit
Intended Use:
This kit is specifically used to determine the percentage and absolute counts of human mature lymphocyte subsets in whole blood samples after red blood cell lysis. It is essential for identifying T lymphocytes (CD3+), helper/inducer T lymphocytes (CD3+CD4+), and suppressor/cytotoxic T lymphocytes (CD3+CD8+). The kit is a valuable tool for immunophenotyping and monitoring immune responses in clinical and research settings.
Product Introduction

The CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Fluorescent Monoclonal Antibody Kit is a state-of-the-art tool for flow cytometry analysis. It enables researchers and clinicians to accurately identify and quantify specific lymphocyte subsets in whole blood samples. The kit includes a combination of monoclonal antibodies conjugated with fluorescent dyes, allowing for simultaneous detection of multiple cell markers. This product is widely used in immunology research, clinical diagnostics, and therapeutic monitoring.

1. The kit features high specificity and sensitivity, ensuring accurate detection of lymphocyte subsets. The antibodies are carefully selected and conjugated with fluorescent dyes to minimize cross-reactivity and background noise.
2. The composition includes CD3-FITC, CD8-PE, CD45-PerCP, and CD4-APC monoclonal antibodies. These antibodies are designed to bind specifically to their respective antigens on the surface of lymphocytes, enabling precise identification of cell populations.
3. The primary use of this kit is to determine the percentage and absolute counts of T lymphocytes (CD3+), helper/inducer T lymphocytes (CD3+CD4+), and suppressor/cytotoxic T lymphocytes (CD3+CD8+) in whole blood samples. It is an essential tool for immunophenotyping and immune response monitoring.

The CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Fluorescent Monoclonal Antibody Kit, also known as the Flow Cytometry Antibody Kit, is a versatile and reliable tool for analyzing lymphocyte subsets. Its high specificity and sensitivity make it indispensable for both research and clinical applications. Whether you're studying immune responses or diagnosing immune disorders, this kit provides the precision and accuracy you need.

Main Features

This kit offers high specificity and sensitivity for detecting lymphocyte subsets, making it a reliable tool for flow cytometry analysis. It includes a combination of monoclonal antibodies conjugated with fluorescent dyes, enabling simultaneous detection of multiple cell markers.

1. High specificity: The antibodies in this kit are designed to bind specifically to their respective antigens, ensuring accurate identification of lymphocyte subsets.
2. Multi-color detection: The kit allows for simultaneous detection of CD3, CD8, CD45, and CD4 markers using different fluorescent dyes, enabling comprehensive analysis of lymphocyte populations.
3. Easy to use: The kit comes with detailed instructions, making it suitable for both experienced researchers and beginners in flow cytometry.
4. Reliable performance: The antibodies are rigorously tested for quality and performance, ensuring consistent and reproducible results.
5. Wide applications: This kit is suitable for a variety of applications, including immunophenotyping, immune response monitoring, and clinical diagnostics.

The CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Fluorescent Monoclonal Antibody Kit stands out for its high specificity, multi-color detection capabilities, and ease of use. Whether you're conducting research or performing clinical diagnostics, this kit delivers reliable and consistent results, making it a valuable addition to your laboratory.

Clinical Applications

This kit is widely used in clinical settings for immunophenotyping and monitoring immune responses. It is particularly useful for diagnosing immune disorders and evaluating the effectiveness of immunotherapies.

1. Immunophenotyping: The kit is used to identify and quantify lymphocyte subsets in patients with immune disorders, such as HIV/AIDS and autoimmune diseases.
2. Immune response monitoring: Clinicians use this kit to monitor changes in lymphocyte populations during immunotherapy or after organ transplantation.
3. Diagnostic tool: The kit is essential for diagnosing conditions characterized by abnormal lymphocyte counts, such as leukemia and lymphoma.
4. Research applications: Researchers use this kit to study the role of lymphocyte subsets in various diseases and to develop new therapeutic strategies.
5. Therapeutic monitoring: The kit is used to assess the effectiveness of immunotherapies and to adjust treatment plans based on changes in lymphocyte populations.

The CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Fluorescent Monoclonal Antibody Kit is a versatile tool in clinical diagnostics and research. Its ability to accurately identify and quantify lymphocyte subsets makes it invaluable for diagnosing immune disorders, monitoring immune responses, and developing new therapies.

Purchasing Guide

When purchasing this kit, consider factors such as product specifications, types of antibodies included, budget, and intended applications. Ensure that the kit meets your specific research or clinical needs.

1. Product specifications: Check the specific antibodies and fluorescent dyes included in the kit to ensure they match your experimental requirements.
2. Product types: Consider whether you need a kit for human or animal samples, as well as the specific lymphocyte subsets you want to analyze.
3. Budget: Compare prices from different suppliers and consider the cost per test to ensure you get the best value for your budget.
4. Usage scenarios: Determine whether the kit will be used for research, clinical diagnostics, or therapeutic monitoring, and choose a kit that is optimized for your intended application.

Purchasing the right CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Fluorescent Monoclonal Antibody Kit requires careful consideration of product specifications, types, budget, and intended applications. By taking these factors into account, you can ensure that you select a kit that meets your specific needs and delivers reliable results.

 Precautions

When using this kit, it is important to follow the manufacturer's instructions carefully to ensure accurate and reliable results. Proper handling and storage of the antibodies are also crucial to maintain their performance.

1. Storage conditions: Store the antibodies at the recommended temperature to prevent degradation and maintain their performance.
2. Handling: Avoid repeated freeze-thaw cycles and handle the antibodies with care to prevent contamination.
3. Sample preparation: Ensure that blood samples are properly collected and processed to avoid artifacts in the flow cytometry analysis.
4. Instrument calibration: Regularly calibrate your flow cytometer to ensure accurate detection of fluorescent signals.
5. Data interpretation: Use appropriate gating strategies and controls to accurately interpret the flow cytometry data.

Using the CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Fluorescent Monoclonal Antibody Kit requires attention to detail and adherence to best practices. By following the manufacturer's instructions and taking proper precautions, you can ensure that your results are accurate and reliable, whether you're conducting research or performing clinical diagnostics.

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