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CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Detection Kit (Flow Cytometry Method)
This kit is composed of four fluorescently labeled monoclonal antibodies: CD3-FITC, CD8-PE, CD45-PerCP, and CD4-APC. It also includes phosphate buffer, protein stabilizers, and preservatives. The detailed composition can be found in the product manual.
Product English Name:
CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Detection Kit (Flow Cytometry Method)
Alias:
T Cell Subset Detection Kit,Flow Cytometry Antibody Kit
Intended Use:
This product is designed for the in vitro quantitative detection of T lymphocytes, T helper/inducer lymphocytes, and cytotoxic T lymphocytes in human peripheral blood samples after red blood cell lysis. It is widely used in immunology research and clinical diagnostics.
Product Introduction

The CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Detection Kit is a specialized tool for flow cytometry, enabling precise identification and quantification of specific T cell subsets in human blood. It is essential for immunophenotyping and understanding immune responses in various diseases.

1. The kit features four highly specific monoclonal antibodies, each conjugated with distinct fluorescent dyes (FITC, PE, PerCP, APC) for simultaneous multi-parameter analysis. This allows for accurate differentiation of T cell subsets.
2. The composition includes optimized reagents such as phosphate buffer and protein stabilizers, ensuring sample integrity and consistent performance during flow cytometry analysis.
3. Its primary use is in immunology research and clinical diagnostics, particularly for monitoring immune cell populations in conditions like HIV, autoimmune diseases, and cancer.

The CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Detection Kit, also known as the T Cell Subset Detection Kit, is a reliable and efficient tool for flow cytometry. Its unique combination of antibodies and stabilizers makes it indispensable for researchers and clinicians studying immune responses.

Main Features

This kit enables simultaneous detection of multiple T cell subsets using fluorescently labeled antibodies, providing high specificity and sensitivity for flow cytometry analysis.

1. The kit includes four monoclonal antibodies, each targeting specific T cell markers (CD3, CD8, CD45, CD4) with distinct fluorescent labels for clear differentiation.
2. It features a phosphate buffer system that maintains sample stability and ensures accurate results during flow cytometry.
3. The protein stabilizers and preservatives enhance the longevity of the reagents, making the kit suitable for long-term studies.
4. Designed for ease of use, the kit simplifies the process of immunophenotyping, reducing preparation time and improving workflow efficiency.
5. Its high reproducibility and consistency make it a trusted choice for both research and clinical applications.

The CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Detection Kit stands out for its precision, reliability, and user-friendly design. Whether you're studying immune responses or diagnosing diseases, this kit delivers consistent and accurate results.

Clinical Applications

This kit is widely used in clinical settings for immunophenotyping, aiding in the diagnosis and monitoring of diseases such as HIV, autoimmune disorders, and cancers.

1. In HIV research, the kit helps quantify CD4+ T cells, a critical parameter for assessing disease progression and treatment efficacy.
2. For autoimmune diseases, it enables the identification of abnormal T cell populations, providing insights into disease mechanisms.
3. In oncology, the kit is used to analyze tumor-infiltrating lymphocytes, aiding in immunotherapy research.
4. It is also employed in transplant medicine to monitor immune cell recovery post-transplant.
5. The kit supports vaccine development by evaluating immune responses in clinical trials.

The CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Detection Kit is a versatile tool in clinical applications, offering valuable insights into immune system dynamics. Its role in diagnosing and monitoring diseases underscores its importance in modern medicine.

Purchasing Guide

When purchasing this kit, consider factors such as product specifications, intended use, budget, and compatibility with your flow cytometry system.

1. Product parameters: Ensure the kit includes all necessary components (antibodies, buffer, stabilizers) and check the fluorescence compatibility with your flow cytometer.
2. Product types: Choose between research-grade and clinical-grade kits based on your application requirements.
3. Budget: Compare prices from different suppliers while considering the kit's performance and reliability.
4. Usage scenarios: Evaluate the kit's suitability for your specific research or diagnostic needs, such as immunophenotyping or disease monitoring.

Purchasing the CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Detection Kit requires careful consideration of your specific needs. By focusing on product quality, compatibility, and budget, you can ensure optimal results for your research or clinical work.

 Precautions

Proper handling and storage of the kit are essential to maintain its performance and ensure accurate results.

1. Store the kit at the recommended temperature (usually 2-8°C) to preserve antibody activity and reagent stability.
2. Avoid repeated freeze-thaw cycles, as this can degrade the fluorescent labels and reduce sensitivity.
3. Follow the manufacturer's instructions for sample preparation and staining to minimize variability and ensure reproducibility.
4. Use appropriate controls, such as unstained and single-stained samples, to validate your flow cytometry results.
5. Dispose of reagents and samples according to local regulations for biohazardous materials.

The CD3-FITC/CD8-PE/CD45-PerCP/CD4-APC Detection Kit is a powerful tool, but its effectiveness depends on proper handling and adherence to protocols. By following these precautions, you can maximize the kit's performance and achieve reliable results.

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