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CD3-FITC/CD4-PE/CD45-PerCP Fluorescent Monoclonal Antibody Kit (Flow Cytometry Method)
The CD3-FITC/CD4-PE/CD45-PerCP Fluorescent Monoclonal Antibody Kit is designed for the detection and quantification of specific lymphocyte subsets in human whole blood samples. It includes antibodies conjugated with fluorescent dyes, enabling precise identification of T lymphocytes (CD3+) and helper/inducer T lymphocytes (CD3+CD4+). The kit is optimized for use with flow cytometry, ensuring high sensitivity and specificity in clinical and research settings.
Product English Name:
CD3-FITC/CD4-PE/CD45-PerCP Fluorescent Monoclonal Antibody Kit (Flow Cytometry Method)
Alias:
Flow Cytometry Antibody Kit,T-Cell Subset Detection Kit
Intended Use:
This kit is primarily used to determine the percentage and absolute counts of mature lymphocyte subsets in lysed whole blood samples. It specifically targets T lymphocytes (CD3+) and helper/inducer T lymphocytes (CD3+CD4+), providing critical data for immunophenotyping and immune system analysis. It is widely applied in clinical diagnostics, immunology research, and therapeutic monitoring.
Product Introduction

The CD3-FITC/CD4-PE/CD45-PerCP Fluorescent Monoclonal Antibody Kit is a state-of-the-art tool for flow cytometry-based immunophenotyping. It enables researchers and clinicians to accurately identify and quantify specific lymphocyte subsets, such as T lymphocytes and helper/inducer T cells, in human blood samples. This kit is essential for understanding immune system dynamics and diagnosing immune-related disorders.

1. The kit features high-quality monoclonal antibodies conjugated with fluorescent dyes (FITC, PE, and PerCP), ensuring bright and stable signals for precise cell population analysis.
2. Its composition includes CD3-FITC, CD4-PE, and CD45-PerCP antibodies, which are specifically designed to target and bind to their respective antigens on lymphocyte surfaces.
3. The primary use of this kit is to determine the percentage and absolute counts of T lymphocytes (CD3+) and helper/inducer T lymphocytes (CD3+CD4+) in lysed whole blood samples, aiding in immunophenotyping and immune system evaluation.

The CD3-FITC/CD4-PE/CD45-PerCP Fluorescent Monoclonal Antibody Kit, also known as the Flow Cytometry Antibody Kit, is a reliable and efficient tool for immune cell analysis. Its ability to accurately identify and quantify specific lymphocyte subsets makes it indispensable in both clinical and research settings.

Main Features

This kit offers precise detection and quantification of lymphocyte subsets using flow cytometry. Its key features include high specificity, stability, and ease of use.

1. High specificity: The monoclonal antibodies are designed to bind exclusively to their target antigens, ensuring accurate cell population identification.
2. Stability: The fluorescent conjugates are stable under various storage and experimental conditions, providing consistent results.
3. Ease of use: The kit includes detailed protocols and is compatible with standard flow cytometry equipment.
4. Comprehensive analysis: It enables simultaneous detection of multiple lymphocyte subsets, streamlining immunophenotyping workflows.
5. Clinical relevance: The kit is validated for use in clinical diagnostics, providing reliable data for immune system assessment.

The CD3-FITC/CD4-PE/CD45-PerCP Fluorescent Monoclonal Antibody Kit stands out for its precision, stability, and user-friendly design. Its ability to deliver reliable and comprehensive immune cell analysis makes it a valuable tool in both research and clinical applications.

Clinical Applications

This kit is widely used in clinical diagnostics and research for immunophenotyping, immune system monitoring, and disease diagnosis.

1. Immunophenotyping: It is used to identify and quantify specific lymphocyte subsets in blood samples, aiding in the diagnosis of immune disorders.
2. Disease monitoring: The kit helps track changes in lymphocyte populations during disease progression or treatment.
3. Research applications: It is employed in studies exploring immune system dynamics and responses.
4. Therapeutic monitoring: Clinicians use the kit to assess the effectiveness of immunotherapies.
5. Diagnostic tool: It provides critical data for diagnosing conditions such as HIV/AIDS, autoimmune diseases, and immunodeficiencies.

The CD3-FITC/CD4-PE/CD45-PerCP Fluorescent Monoclonal Antibody Kit is a versatile tool with broad clinical and research applications. Its ability to provide detailed immune cell analysis makes it indispensable for diagnosing and monitoring immune-related conditions.

Purchasing Guide

When purchasing this kit, consider factors such as product specifications, intended use, budget, and compatibility with existing equipment.

1. Product parameters: Ensure the kit meets your specific requirements for antibody specificity, fluorescence intensity, and stability.
2. Product types: Choose between research-grade and clinical-grade kits based on your application needs.
3. Budget: Evaluate the cost-effectiveness of the kit, considering its performance and the volume of samples to be processed.
4. Usage scenarios: Assess whether the kit is suitable for your laboratory setup and workflow.

Purchasing the CD3-FITC/CD4-PE/CD45-PerCP Fluorescent Monoclonal Antibody Kit requires careful consideration of its specifications, intended use, and compatibility with your laboratory setup. Making an informed decision ensures optimal performance and value.

 Precautions

Proper handling and storage of the kit are essential to maintain its performance and ensure accurate results.

1. Storage: Store the kit at the recommended temperature to preserve antibody stability.
2. Handling: Avoid repeated freeze-thaw cycles and protect the antibodies from light.
3. Experimental conditions: Follow the protocol carefully to ensure optimal staining and analysis.
4. Safety: Use appropriate personal protective equipment when handling blood samples and reagents.
5. Quality control: Include controls in each experiment to validate the results.

The CD3-FITC/CD4-PE/CD45-PerCP Fluorescent Monoclonal Antibody Kit requires careful handling and storage to maintain its performance. Adhering to the recommended precautions ensures reliable and accurate results in your experiments.

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