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CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC Detection Kit (Flow Cytometry Method)
This kit is primarily composed of CD3, CD8, CD45, and CD4 detection reagents, along with a lysing agent. These components are specifically designed for use in flow cytometry to identify and quantify specific T-cell subsets in human blood samples. The reagents are optimized for high sensitivity and specificity, ensuring accurate results in clinical and research settings.
Product English Name:
CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC Detection Kit (Flow Cytometry Method)
Alias:
T-Cell Subset Detection Kit,Flow Cytometry Reagent Kit
Intended Use:
This product is intended for the in vitro quantitative detection of T-lymphocytes (CD3+), helper/inducer T-cells (CD3+CD4+), and suppressor/cytotoxic T-cells (CD3+CD8+) in human venous whole blood samples. It is widely used in immunology research, clinical diagnostics, and monitoring of immune system disorders.
Product Introduction

The CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC Detection Kit is a state-of-the-art tool designed for flow cytometry analysis. It enables precise identification and quantification of T-cell subsets in human blood, providing critical insights into immune system function. This kit is essential for researchers and clinicians studying immune responses, autoimmune diseases, and immunodeficiency conditions.

1. The kit features highly specific antibodies conjugated to fluorescent dyes, ensuring accurate detection of CD3, CD8, CD45, and CD4 markers. These markers are crucial for distinguishing different T-cell populations.
2. The reagents are formulated to work seamlessly with flow cytometry instruments, offering high reproducibility and minimal background noise. The inclusion of a lysing agent simplifies sample preparation, making the process efficient and user-friendly.
3. This kit is widely used in clinical diagnostics to monitor immune system health, assess the efficacy of immunotherapies, and study the progression of diseases such as HIV/AIDS and cancer.

The CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC Detection Kit is a reliable and versatile tool for flow cytometry. Its ability to accurately identify T-cell subsets makes it indispensable in both research and clinical settings. Whether you're studying immune responses or diagnosing immune disorders, this kit delivers consistent and precise results.

Main Features

This detection kit offers high sensitivity and specificity for identifying T-cell subsets, making it a valuable tool for immunology research and clinical diagnostics.

1. The kit includes antibodies conjugated to fluorescent dyes (FITC, PE, PerCP, and APC), enabling simultaneous detection of multiple T-cell markers.
2. It is optimized for use with flow cytometry instruments, ensuring high reproducibility and minimal cross-reactivity.
3. The included lysing agent simplifies sample preparation, reducing processing time and improving workflow efficiency.
4. The kit is designed for use with human venous whole blood, making it suitable for a wide range of clinical and research applications.
5. It provides accurate quantification of T-cell subsets, aiding in the diagnosis and monitoring of immune-related diseases.

The CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC Detection Kit stands out for its precision and ease of use. Its ability to simultaneously detect multiple T-cell markers makes it a powerful tool for understanding immune system dynamics and diagnosing immune disorders.

Clinical Applications

This kit is widely used in clinical settings to monitor immune system health, diagnose immune disorders, and evaluate the effectiveness of immunotherapies.

1. It is used to assess T-cell subsets in patients with HIV/AIDS, providing critical information about disease progression and treatment efficacy.
2. The kit is employed in cancer research to study the role of T-cells in tumor immunity and the effectiveness of immunotherapies.
3. It aids in the diagnosis of autoimmune diseases by quantifying abnormal T-cell populations.
4. The kit is used in transplant medicine to monitor immune responses and detect graft rejection.
5. It is also utilized in vaccine development to evaluate immune responses and optimize vaccine formulations.

The CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC Detection Kit is a versatile tool in clinical applications. Its ability to provide detailed insights into T-cell populations makes it invaluable for diagnosing and managing a wide range of immune-related conditions.

Purchasing Guide

This guide provides essential information for purchasing the CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC Detection Kit, including product specifications, types, budget considerations, and usage scenarios.

1. Product parameters: Ensure the kit includes all necessary reagents (CD3 FITC, CD8 PE, CD45 PerCP, CD4 APC) and a lysing agent. Verify compatibility with your flow cytometry instrument.
2. Product types: Choose between research-grade and clinical-grade kits based on your intended application. Clinical-grade kits are validated for diagnostic use.
3. Budget considerations: Compare prices from different suppliers, but prioritize quality and reliability. Consider bulk purchasing for cost savings.
4. Usage scenarios: Evaluate the kit's suitability for your specific needs, such as research, clinical diagnostics, or monitoring immune therapies.

Purchasing the right CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC Detection Kit requires careful consideration of product specifications, intended use, and budget. By selecting a high-quality kit that meets your needs, you can ensure accurate and reliable results in your work.

 Precautions

Proper handling and storage of the CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC Detection Kit are essential to ensure accurate results and maintain reagent stability.

1. Store the kit at the recommended temperature (usually 2-8°C) and protect it from light to prevent degradation of fluorescent dyes.
2. Follow the manufacturer's instructions for sample preparation and reagent handling to avoid contamination or inaccurate results.
3. Use appropriate personal protective equipment (PPE) when handling blood samples and reagents to ensure safety.
4. Calibrate your flow cytometry instrument regularly to ensure accurate detection and quantification of T-cell subsets.
5. Dispose of used reagents and samples according to local regulations and biohazard waste disposal guidelines.

Taking proper precautions when using the CD3 FITC/CD8 PE/CD45 PerCP/CD4 APC Detection Kit is crucial for obtaining reliable results and ensuring safety. By following the recommended guidelines, you can maximize the kit's performance and minimize risks.

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